Biochemical study of individual zonae from human oocytes that failed to undergo fertilization in intracytoplasmic sperm injection
نویسندگان
چکیده
منابع مشابه
Failed fertilization after clinical intracytoplasmic sperm injection.
Intracytoplasmic sperm injection (ICSI) has resulted in pregnancy and birth for many couples, including those with severe male factor infertility. However, even after ICSI, complete failure of fertilization occurs in 1-3% of cycles. Most cases occur due to low number of mature oocytes, failure of oocyte activation or non-availability of appropriate spermatozoa for injection. Given the significa...
متن کاملIn vitro fertilization by intracytoplasmic sperm injection.
Intracytoplasmic sperm injection (ICSI) is the latest, and by far the most efficient, variant of micromanipulation-assisted fertilization, whereby a single spermatozoon is selected, aspirated into a microinjection needle and injected to the oocyte cytoplasm. The development of this technique is mainly linked to application in human assisted reproduction for which it enables fertilization with d...
متن کاملPreincubation of human oocytes may improve fertilization and embryo quality after intracytoplasmic sperm injection.
The aim of this study was to examine the relationship between different preincubation periods of oocytes and the outcome of intracytoplasmic sperm injection (ICSI). We analysed retrospectively 95 ICSI treatment cycles performed to alleviate severe male-factor infertility. Oocyte collection was performed approximately 36 h after human chorionic gonadotrophin administration. The cumulus-corona-oo...
متن کاملApoptosis in human unfertilized oocytes after intracytoplasmic sperm injection.
OBJECTIVE To investigate the presence of programmed cell death in unfertilized oocytes after intracytoplasmic sperm injection (ICSI), assuming that previous apoptotic events could be correlated with the fertilization failure. DESIGN Comparison of the rate of DNA fragmentation in human oocytes at different stages of maturation soon after pick-up (control) and in unfertilized oocytes after ICSI...
متن کاملFertilization and pregnancy potential of immature oocytes from stimulated intracytoplasmic sperm injection cycles
OBJECTIVE We evaluated the fertilization potential of immature oocytes obtained from controlled ovarian hyperstimulation cycles of patients undergoing ICSI. METHODS We retrospectively analyzed 463 ICSI cycles containing at least one immature oocyte at oocyte denudation. ICSI was performed on mature oocytes at oocyte denudation (metaphase-II [MII] oocytes) and the oocytes that extruded the fir...
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ژورنال
عنوان ژورنال: Molecular Human Reproduction
سال: 1996
ISSN: 1360-9947,1460-2407
DOI: 10.1093/molehr/2.12.959